By 1843, Ota-juku was about 680 meters long with one Honjin, one Waki-Honjin (partially open to the public), 3 toiyaba, 20 hatago inns, and 118 homes. 505 people lived in Ota-juku.

A major highlight was when Princess Kazunomiya (1846–1877) arrived and stayed in Ota-juku on Oct. 27, 1861 on her way to Edo/Tokyo to marry the shogun. She had such a huge entourage that it took them 4 days to pass through Ota-juku.